Acute and Repeated-Doses (28 Days) Toxicity of Thymol Formulation in Male Albino Rats

The objective of this study was to evaluate the acute and repeated-doses toxicity (28 days) of thymol oil-water emulsion formulation via oral route in male albino rats. For the acute toxicity study, thymol formulation administrated to rats by oral route at doses ranging from 500 to 4000 mg/kgbw. General behavior, adverse effects, mortality were recorded for up to 14 days post treatment. In the repeated-doses toxicity study, thymol formulation was administrated orally at doses of 15.39, 30.78 and 61.55 mg/kgbw for 28 days to rats. Animal body weight was observed weekly. In the acute toxicity study, the median lethal dose (LD50) of thymol formulation was found to be 2462.23 mg/kgbw. In repeated-doses toxicity study, thymol formulation at different doses used, did not induce any statistically significant changes in body weight gain or organs weight compared to control group. It is also showed no significant alteration in haematological and biochemical parameters in treatment groups. Histopathological analyses, revealed normal architecture of kidney and liver of treatment groups. The no-observed adverse effect level (NOAEL) for repeated-doses administration in thymol formulation was considered to be 61.55 mg/kg body weight.